ReproCELL has developed a human iPS cell-derived dopaminergic neuron kit. The kit includes human iPS cell-derived neural progenitor cells, maturation medium, Additive A and other necessary reagents. Human dopaminergic neurons can be obtained after one week by differentiating the neural progenitor cells using the reagents included in the kit.
Differentiated dopaminergic neurons are confirmed to express both bIII-tubulin (a neuron marker) and TH (a dopaminergic neuron marker). Dopaminergic neuron function was also demonstrated. The figure on the right shows the results of dopamine concentration in the culture medium when the KCl level is increased to 56 mM, which suggests dopamine release from the neurons by KCl-mediated depolarization. Dopamine uptake in the neurons was demonstrated by the Neurotransmitter
Transporter Uptake Assay Kit, and the uptake was suppressed when dopamine transporter (DAT) inhibitor was added. These results suggest that hiPSC-derived dopaminergic neurons possess the ability of both dopamine release and uptake, and function like human dopaminergic neurons.
ReproCELL's human iPSC-derived dopaminergic neurons can be an effective in vitro drug discovery tool for CNS diseases including Parkinson's disease, and for other basic research.
Human dopaminergic neuron Expressing both bIII-tubulin (a neuron marker) and TH (a dopaminergic neuron marker) Dopamine release and uptake are observed All lots are inspected using functional assays
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Differentiated dopaminergic neurons are confirmed to express both bIII-tubulin (a neuron marker) and TH (a dopaminergic neuron marker). Dopaminergic neuron function was also demonstrated. The figure on the right shows the results of dopamine concentration in the culture medium when the KCl level is increased to 56 mM, which suggests dopamine release from the neurons by KCl-mediated depolarization. Dopamine uptake in the neurons was demonstrated by the Neurotransmitter
Transporter Uptake Assay Kit, and the uptake was suppressed when dopamine transporter (DAT) inhibitor was added. These results suggest that hiPSC-derived dopaminergic neurons possess the ability of both dopamine release and uptake, and function like human dopaminergic neurons.
Human dopaminergic neuron