Publications
HAC Vector
Kazuki Y, Hoshiya H, Takiguchi M, Abe S, Iida Y, Osaki M, Katoh M, Hiratsuka M, Shirayoshi Y, Hiramatsu K, Ueno E, Kajitani N, Yoshino T, Kazuki K, Ishihara C, Takehara S, Tsuji S, Ejima F, Toyoda A, Sakaki Y, Larionov V, Kouprina N, Oshimura M: Refined human artificial chromosome vectors for gene therapy and animal transgenesis. Gene Ther 18:384-393,2011 (PMID: 21085194)
Yamaguchi S, Kazuki Y, Nakayama Y, Nanba E, Oshimura M, Ohbayashi T: A method for producing transgenic cells using a multi-integrase system on a human artificial chromosome vector. PLoS One 6:e17267,2011 (PMID: 21390305)
Kazuki Y, Oshimura M: Human artificial chromosomes for gene delivery and the development of animal models. Mol Ther 19:1591-1601,2011 (PMID: 21750534)
Iida Y, Kim JH, Kazuki Y, Hoshiya H, Takiguchi M, Hayashi M, Erliandri I, Lee HS, Samoshkin A, Masumoto H, Earnshaw WC, Kouprina N, Larionov V, Oshimura M: Human artificial chromosome with a conditional centromere for gene delivery and gene expression. DNA Res 17:293-301,2010 (PMID: 20798231)
Otsuki A, Tahimic CG, Tomimatsu N, Katoh M, Chen DJ, Kurimasa A, Oshimura M: Construction of a novel expression system on a human artificial chromosome.. Biochem Biophys Res Commun 329:1018-1025,2005 (PMID: 15752757)
Katoh M, Ayabe F, Norikane S, Okada T, Masumoto H, Horike S, Shirayoshi Y, Oshimura M: Construction of a novel human artificial chromosome vector for gene delivery. Biochem Biophys Res Commun 321:280-290,2004 (PMID: 15358173)
Trans-chromosomic mice and cells
Tedesco FS, Hoshiya H, D'Antona G, Gerli MF, Messina G, Antonini S, Tonlorenzi R, Benedetti S, Berghella L, Torrente Y, Kazuki Y, Bottinelli R, Oshimura M, Cossu G: Stem cell-mediated transfer of a human artificial chromosome ameliorates muscular dystrophy. Sci Transl Med 3:96ra78,2011 (PMID: 21849666)
Kazuki Y, Hiratsuka M, Takiguchi M, Osaki M, Kajitani N, Hoshiya H, Hiramatsu K, Yoshino T, Kazuki K, Ishihara C, Takehara S, Higaki K, Nakagawa M, Takahashi K, Yamanaka S, Oshimura M: Complete genetic correction of ips cells from Duchenne muscular dystrophy. Mol Ther 18:386-393,2010 (PMID: 19997091)
Hoshiya H, Kazuki Y, Abe S, Takiguchi M, Kajitani N, Watanabe Y, Yoshino T, Shirayoshi Y, Higaki K, Messina G, Cossu G, Oshimura M: A highly stable and nonintegrated human artificial chromosome (HAC) containing the 2.4 Mb entire human dystrophin gene. Mol Ther 17:309-317,2009 (PMID: 19034264)
Kazuki Y, Kimura M, Nishigaki R, Kai Y, Abe S, Okita C, Shirayoshi Y, Schulz TC, Tomizuka K, Hanaoka K, Inoue T, Oshimura M: Human chromosome 21q22.2-qter carries a gene(s) responsible for downregulation of mlc2a and PEBP in Down syndrome model mice. Biochem Biophys Res Commun 317:491-499,2004 (PMID: 15063784)
Kuroiwa Y, Yoshida H, Ohshima T, Shinohara T, Ohguma A, Kazuki Y, Oshimura M, Ishida I, Tomizuka K: The use of chromosome-based vectors for animal transgenesis.. Gene Ther 9:708-712,2002 (PMID: 12032693)
Shinohara T, Tomizuka K, Miyabara S, Takehara S, Kazuki Y, Inoue J, Katoh M, Nakane H, Iino A, Ohguma A, Ikegami S, Inokuchi K, Ishida I, Reeves RH, Oshimura M: Mice containing a human chromosome 21 model behavioral impairment and cardiac anomalies of Down's syndrome.. Hum Mol Genet 10:1163-1175,2001 (PMID: 11371509)
Kazuki Y, Shinohara T, Tomizuka K, Katoh M, Ohguma A, Ishida I, Oshimura M: Germline transmission of a transferred human chromosome 21 fragment in transchromosomal mice. J Hum Genet 46:600-603,2001 (PMID: 11587075)
Tomizuka K, Shinohara T, Yoshida H, Uejima H, Ohguma A, Tanaka S, Sato K, Oshimura M, Ishida I: Double trans-chromosomic mice: maintenance of two individual human chromosome fragments containing Ig heavy and kappa loci and expression of fully human antibodies. Proc Natl Acad Sci U S A 97:722-727,2000 (PMID: 10639146)
Kuroiwa Y, Tomizuka K, Shinohara T, Kazuki Y, Yoshida H, Ohguma A, Yamamoto T, Tanaka S, Oshimura M, Ishida I: Manipulation of human minichromosomes to carry greater than megabase-sized chromosome inserts. Nat Biotechnol 18:1086-1090,2000 (PMID: 11017048)
Shinohara T, Tomizuka K, Takehara S, Yamauchi K, Katoh M, Ohguma A, Ishida I, Oshimura M: Stability of transferred human chromosome fragments in cultured cells and in mice. Chromosome Res 8:713-725,2000 (PMID: 11196134)
Tomizuka K, Yoshida H, Uejima H, Kugoh H, Sato K, Ohguma A, Hayasaka M, Hanaoka K, Oshimura M, Ishida I: Functional expression and germline transmission of a human chromosome fragment in chimaeric mice. Nat Genet 16:133-143,1997 (PMID: 9171824)
Gene Expression
Kurosaki H, Hiratsuka M, Imaoka N, Iida Y, Uno N, Kazuki Y, Ishihara C, Yakura Y, Mimuro J, Sakata Y, Takeya H, Oshimura M: Integration-free and stable expression of FVIII using a human artificial chromosome. J Hum Genet. 2011 Aug 11. doi: 10.1038/jhg.2011.88.,2011 (PMID: 21833006)
Yamada H, Li YC, Nishikawa M, Oshimura M, Inoue T: Introduction of a CD40L genomic fragment via a human artificial chromosome vector permits cell-type-specific gene expression and induces immunoglobulin secretion. J Hum Genet 53:447-453,2008 (PMID: 18322642)
Kawahara M, Inoue T, Ren X, Sogo T, Yamada H, Katoh M, Ueda H, Oshimura M, Nagamune T: Antigen-mediated growth control of hybridoma cells via a human artificial chromosome. Biochim Biophys Acta 1770:206-212,2007 (PMID: 17184921)
Suda T, Katoh M, Hiratsuka M, Takiguchi M, Kazuki Y, Inoue T, Oshimura M: Heat-regulated production and secretion of insulin from a human artificial chromosome vector. Biochem Biophys Res Commun 340:1053-1061,2006 (PMID: 16403445)
Yamada H, Kunisato A, Kawahara M, Tahimic CG, Ren X, Ueda H, Nagamune T, Katoh M, Inoue T, Nishikawa M, Oshimura M: Exogenous gene expression and growth regulation of hematopoietic cells via a novel human artificial chromosome. J Hum Genet 51:147-150,2006 (PMID: 16333524)
Kakeda M, Hiratsuka M, Nagata K, Kuroiwa Y, Kakitani M, Katoh M, Oshimura M, Tomizuka K: Human artificial chromosome (HAC) vector provides long-term therapeutic transgene expression in normal human primary fibroblasts.. Gene Ther 12:852-856,2005 (PMID: 15750614)
Ayabe F, Katoh M, Inoue T, Kouprina N, Larionov V, Oshimura M: A novel expression system for genomic DNA loci using a human artificial chromosome vector with transformation-associated recombination cloning. J Hum Genet 50:592-599,2005 (PMID: 16231070)
Ren X, Katoh M, Hoshiya H, Kurimasa A, Inoue T, Ayabe F, Shibata K, Toguchida J, Oshimura M: A novel human artificial chromosome vector provides effective cell lineage-specific transgene expression in human mesenchymal stem cells.. Stem Cells 23:1608-1616,2005 (PMID: 16141362)
Technologies
Katoh M, Kazuki Y, Kazuki K, Kajitani N, Takiguchi M, Nakayama Y, Nakamura T, Oshimura M: Exploitation of the interaction of measles virus fusogenic envelope proteins with the surface receptor CD46 on human cells for microcell-mediated chromosome transfer. BMC Biotechnol 10:37,2010 (PMID: 20444293)
Kuroiwa Y, Shinohara T, Notsu T, Tomizuka K, Yoshida H, Takeda S, Oshimura M, Ishida I: Efficient modification of a human chromosome by telomere-directed truncation in high homologous recombination-proficient chicken DT40 cells. Nucleic Acids Res 26:3447-3448,1998 (PMID: 9649633)
Koi M, Shimizu M, Morita H, Yamada H, Oshimura M: Construction of mouse A9 clones containing a single human chromosome tagged with neomycin-resistance gene via microcell fusion. Jpn J Cancer Res 80:413-418,1989 (PMID: 2502516)
HTS System
Yasunaga M, Oumi N, Osaki M, Kazuki Y, Nakanishi T, Oshimura M, Sato K: Establishment and characterization of a transgenic mouse model for in vivo imaging of bmp4 expression in the pancreas. PLoS One 6:e24956,2011 (PMID: 21949805)
Takahashi Y, Tsuji S, Kazuki Y, Noguchi M, Arifuku I, Umebayashi Y, Nakanishi T, Oshimura M, Sato K: Development of evaluation system for bioactive substances using human artificial chromosome-mediated osteocalcin gene expression. J Biochem 148:29-34,2010 (PMID: 20299329)
Regenerative Medicine
Hiratsuka M, Uno N, Ueda K, Kurosaki H, Imaoka N, Kazuki K, Ueno E, Akakura Y, Katoh M, Osaki M, Kazuki Y, Nakagawa M, Yamanaka S, Oshimura M: Integration-Free iPS Cells Engineered Using Human Artificial Chromosome Vectors. PLoS One 6:e25961,2011 (PMID: 21998730)
Kinoshita Y, Kamitani H, Mamun MH, Wasita B, Kazuki Y, Hiratsuka M, Oshimura M, Watanabe T: A gene delivery system with a human artificial chromosome vector based on migration of mesenchymal stem cells towards human glioblastoma HTB14 cells. Neurol Res 32:429-437,2010 (PMID: 19589205)
Shitara S, Kakeda M, Nagata K, Hiratsuka M, Sano A, Osawa K, Okazaki A, Katoh M, Kazuki Y, Oshimura M, Tomizuka K: Telomerase-mediated life-span extension of human primary fibroblasts by human artificial chromosome (HAC) vector.. Biochem Biophys Res Commun 369:807-811,2008 (PMID: 18328257)
Kazuki Y, Hoshiya H, Kai Y, Abe S, Takiguchi M, Osaki M, Kawazoe S, Katoh M, Kanatsu-Shinohara M, Inoue K, Kajitani N, Yoshino T, Shirayoshi Y, Ogura A, Shinohara T, Barrett JC, Oshimura M: Correction of a genetic defect in multipotent germline stem cells using a human artificial chromosome. Gene Ther 15:617-624,2008 (PMID: 18305578)
Oshimura M, Katoh M: Transfer of human artificial chromosome vectors into stem cells. Reprod Biomed Online 16:57-69,2008 (PMID: 18252049)
Ren X, Tahimic CG, Katoh M, Kurimasa A, Inoue T, Oshimura M: Human artificial chromosome vectors meet stem cells: new prospects for gene delivery. Stem Cell Rev 2:43-50,2006 (PMID: 17142886)